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موضوع به انگلیسی:Application of the protoplast co-culture method for evaluation of
allelopathic activities of volatile compounds, safranal and tulipalin A
بخشی از متن:The effects of two plant volatile compounds (VOCs), safranal and tulipalin A were investigated using the protoplast
method with digital image analysis, which was developed to investigate allelopathic activities of plants at
the cellular level in a 50 μL liquid medium. Both VOCs showed inhibition on lettuce protoplasts growth at all
three stages, i.e., the cell wall formation stage, the cell division stage, and the yellow colour accumulation
stage. Among the three stages of protoplast growth, differences of inhibition patternswere observed. Inhibitions
at the cellwall formation stagewere stronger than those at the latter two stages in both VOCs. Tulipalin A showed
higher inhibitory activity (total inhibition at 100 μM) than that of safranal (44% inhibition at 1 mM) at cell division
stage. They were also compared with the direct exposuremethod in an enclosed vial using germinated lettuce
seedlings, which was developed to investigate allelopathic activities of VOCs at plant level. Tulipalin A
showed less inhibitory activity on radicle growth of lettuce, than that of safranal reported. Inhibition on hypocotyl
growthwas stronger than that on radicle in both VOCs. The causes of the differences between two VOCs and two
bioassay methods, and the application of the protoplast method,were discussed for clarifying the contribution of
VOCs as allelochemicals at cellular level
