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موضوع به انگلیسی:Steps toward enhancing the fluorescence of small-molecule-based protein
labels using supramolecular hosts
بخشی از متن:Labeling of proteins with small‐molecule‐based fluorescent probes provides a powerful tool to determine
their expression level and localization in living cells. We propose a method for increasing and stabilizing
the emission of such probes using supramolecular hosts such as β‐cyclodextrin (β‐CD). Although the emission
of a His‐tag binding probe was enhanced in the presence of β‐CD, binding of the probe‐β‐CD complex
to a His‐tagged protein led to partial β‐CD displacement and consequently, a reduction in the emission
signal
