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موضوع به انگلیسی:A proposed mechanism to induce multi-layer polydiacetylene-coated
filter color response to bacteria
بخشی از متن:The purpose of this studywas to develop a multi-layer diacetylene (PDA)-coated filter and to verify the proposed
color response mechanism of PDA-coated filters to bacteria. Unlike other reports which immobilize PDA liposomes
on solid support or create monolayer Langmuir films, multi-layer PDA-coated filters were generated by
directly evaporating organic solvents. Different from incorporating functional headgroups or inserting phospholipids
into PDA, we reported that bacterial growth can trigger the color change of PDA sensorswithout anymodification
or phospholipid insertion. The mechanism that pH change from bacteria metabolites lead to color
change of PDA filterwas proposed and verified by carefully designed dextrose-freemediumwith phenol red. Further,
culturing Salmonella Typhimurium, E. coli, L. innocua andM. luteuswith PDA-coated filters on phenol red agar
in absence of dextrose verified the potential of applying PDA-coated filter for bacterial detection, specifically
amine-producing bacteria. Thus, PDA-coated filtersmay be a useful tool for food safety and shelf life applications.
©2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http