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موضوع به انگلیسی:Easily ignored interference light from the second order diffraction of the
excitation grating in a xenon lamp-based spectrometer
بخشی از متن:Due to the diffraction of light by gratings, use of optical filters is typically recommended in the emission exit in
xenon lamp-based spectrofluorometer, especially for turbid samples. However, such diffraction also exists in
the excitation, but is typically ignored. In this work,we demonstrated that the higher order diffraction of gratings
in the excitation exit in the front of xenon lamp could cause real light, which could interference the fluorescence
detection for NIR-emitting fluorophores. Besides, such interference light may also result in debated upconversion
results in nowadays' nano-fluorophores. Overall, we wanted to call attention for the use of optical filters in the
excitation exit to exclude potential biased results in fluorescence-related research, such as NIR-emissive
fluorophores and long wavelength photocatalysis