دانلود مقاله با موضوع کاوشگر نسبت سنجی دو فوتونی حساس به محیط برای در سلولو اندازه گیری
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موضوع به انگلیسی:Environment-insensitive two-photon ratiometric probe for in cellulo
quantitative measurement of hydrogen peroxide
بخشی از متن:The quantitative analysis of a biological analyte directly through fluorescence imaging is essential in biomedical
sciences but remains as a challenge owing to the environment‐sensitive nature of fluorescence intensity.
We show that a fluorescent hydrogen peroxide probe based on an in cellulo super‐bright benzothiazolylbenzocoumarin
dye of which emission properties are insensitive to environmental changes offers a reliable
and practical means for the quantitative analysis of hydrogen peroxide in cells and tissues directly through
ratiometric imaging both under one‐photon and two‐photon excitation conditions. Different cellular hydrogen
peroxide concentrations were determined for several cell lines and also for tumour and normal tissues