دانلود مقاله با موضوع تخمین پراکسیداز ترب بی حرکت در یک نمک کم غلظت برای یک سیستم الکتروشیمیایی
در قالب pdf و در 7 اسلاید،قابل ویرایش، شامل:
موضوع به انگلیسی:Estimation of immobilized horseradish peroxidase in a low salt
concentration for an irreversible electrochemical system
بخشی از متن:We examined the direct electron transfer of horseradish peroxidase (HRP) at HRP/carbon paste (CP)-modified
electrodes under irreversible electrochemical conditions. The amount of electroactive HRP (Γ) was estimated
as 2.0 × 10−9 to 3.9 × 10−9 mol, indicating that the effectiveness factor, defined as the ratio of the amount of
electroactive HRP to the amount of HRP incorporated into CP, was 0.14–0.24. In the presence of potassium ferricyanide(
III) (K3Fe(CN)6) and potassiumhexacyanoferrate(II) (K4Fe(CN)6) asmediators, the electroactive surface
area of only HRP on the HRP/CP-modified electrodewas 0.0164 cm2. The theoretically calculated coverage area of
electroactive HRP on the HRP/CP-modified electrode was much greater than the electroactive surface area of the
CP electrode. In addition, the rate constant (k°) of the electrochemical reaction of HRP at the HRP/CP-modified
electrode was 0.006 s−1 for the oxidation process.
