دانلود مقاله با موضوع بررسی پتانسیل DNA i-motif تشکیل شده در ناحیه پروموتر GRIN1 ژن
در قالب pdf و در 10 اسلاید،قابل ویرایش، شامل:
موضوع به انگلیسی:Exploring potential of i-motif DNA formed in the promoter region of GRIN1
gene for nanotechnological applications
بخشی از متن:The tandem repeat stretches of cytosine residues fold to construct intercalated motif (i‐motif) structures over an
acidic pH range. Several advancements have been made to understand the biological role of i‐motif DNA.
Potential i‐motif structures found in gene promoters are involved in positive transcriptional regulation and
sequence specific DNA binding. Herein, a 27‐mer C‐rich DNA stretch present at the promoter region of
(Glutamate Ionotropic Receptor NMDA type subunit 1) GRIN1 gene, named GRIN1c was studied to investigate
its structural status using biophysical and biochemical techniques. The gel electrophoresis, circular dichroism
spectroscopy and pH‐dependent monophasic UV‐thermal melting data revealed that GRIN1c sequence adapts a
bimolecular i‐motif structure. Two alternative structural models are suggested for the bimolecular i‐motif structure
with typical C·C+ base pairing and significant Watson‐Crick base pairing. Possibility of co‐existence of
non‐Watson‐Crick and Watson‐Crick base pairing in the formation of i‐motif expands the repertoire of various
topologies of i‐motif structures. Extensive applications of i‐motif structure in the areas of nanotechnology, analytical
and biomedical systems and many more have made it essential to explore these multistranded structures.
Nanodevices‐based on i‐motif structure found applications as biosensors and drug release platform,
and also serve as proof for their in vivo existence
